Real Time 3D fluorescence microscopy based on multifocal multiphoton excitation

نویسنده

  • Ramesh Ahuja
چکیده

Multiphoton excitation microscopy is becoming increasingly important as it offers several advantages over confocal laser (single photon excitation) scanning microscopy (CLSM) such as use of lesser damaging NIR wavelengths, reduced scattering, deeper imaging, no need for pinhole, higher light collection efficiency and excitation of UV dyes at NIR wavelengths; in addition, excitation and photobleaching are limited to the focal region.

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تاریخ انتشار 2003